CyFlow™ ABRA1 Purified

CyFlow™ ABRA1 Purified
Target Species: Human
Antigen: ABRA1
Field of Interest: Adaptor proteins, Transcription
Clone: ABRA1-01
Regulatory Status: RUO
Application: Flow cytometry, Immunoprecipitation, Western blot
Format/Fluorochrome: Purified
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG
Product number: AP667270

For Research Use Only

Concentration 1 mg/mL Quantity 0.1 mg Volume 0.1 mL Immunogen Recombinant protein... more
CyFlow™ ABRA1 Purified
Concentration1 mg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenRecombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag
Background InformationABRA1 (Abraxas, CCDC98) is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007·May·25; 316(5828):1194‑8. <·PMID:·17525340·> | Liu Z, Wu J, Yu X: CCDC98 targets BRCA1 to DNA damage sites. Nat·Struct·Mol·Biol. 2007·Aug; 14(8):716‑20. <·PMID:·17643121·> | Wang B, Elledge SJ: Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage. Proc·Natl·Acad·Sci·USA. 2007·Dec·26; 104(52):20759‑63. <·PMID:·18077395·> | Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA: Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Oncogene. 2009·Apr·23; 28(16):1843‑52. <·PMID:·19305427·>

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