Laser: Blue, Green, Yellow
Target Species: Human
Antigen: ARHGEF4
Field of Interest: Signaling
Clone: ARHGEF-08
Regulatory Status: RUO
Application: Flow cytometry
Format/Fluorochrome: PE
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgG1
Product number: BV459562

For Research Use Only

Concentration 0.1 mg/mL Quantity 0.1 mg Volume 1.0 mL Immunogen Recombinant... more
Concentration0.1 mg/mL
Quantity0.1 mg
Volume1.0 mL
ImmunogenRecombinant fragment of human ARHGEF4 (amino acids 143-271)
Background InformationARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF1 (Adenomatous polyposis coli - stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Available Technical Data Sheets

Specific References

| Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3‑kinase. J·Biol·Chem. 2009·Aug·14; 284(33):22436‑43. <·PMID:·19525225·> | Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL‑AML1 to promote leukaemic transformation. Leuk·Res. 2010·Jan; 34(1):109‑15. <·PMID:·19628279·> | Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell·Res. 2012·Feb; 22(2):372‑86. <·PMID:·21788986·>

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