Article successfully added.

CyFlow™ IFN-gamma PerCP-Cy5.5

CyFlow™ IFN-gamma PerCP-Cy5.5
Antibody: Yes
Antigen: IFN-γ
Application: Flow cytometry
Clonality: monoclonal
Clone: 4S.B3
Emission Maximum: 695 nm
Excitation Maximum: 482 nm
Field of Interest: Signaling
Format/Fluorochrome: PerCP-Cy5.5
Isotype: IgG1
Laser: Blue , Green
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CS240755

For Research Use Only

Quantity 100 tests Volume 0.4 mL Immunogen Interferon γ derived from human leukocytes... more
CyFlow™ IFN-gamma PerCP-Cy5.5
Quantity100 tests
Volume0.4 mL
ImmunogenInterferon γ derived from human leukocytes
Background InformationInterferon γ (IFN-γ) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-α and IL-12; its production is suppressed by IL-4, IL-10, and TGF-β. The production of IFN-γ is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-γ polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-γ belongs to the type II interferons, also called immune IFN. IFN-γ shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-γ plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-γ to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-γ is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumors. The activation of macrophages by IFN-γ is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-γ can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-α, IFN-β). IFN-γ may also activate neutrophils and NK cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Meager A, Parti S, Barwick S, Spragg J, O'Hagan K: Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon. J Interferon Res. 1984 Fall; 4(4):619‑25. < PMID: 6438252 > | Caulfield JJ, Fernandez MH, Sousa AR, Lane SJ, Lee TH, Hawrylowicz CM: Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte‑macrophage colony‑stimulating factor in the presence of glucocorticoids. Immunology. 1999 Sep; 98(1):104‑10. < PMID: 10469240 > | Coles AJ, Wing M, Smith S, Coraddu F, Greer S, Taylor C, Weetman A, Hale G, Chatterjee VK, Waldmann H, Compston A: Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis. Lancet. 1999 Nov 13; 354(9191):1691‑5. < PMID: 10568572 > | Brattig NW, Lepping B, Timmann C, Büttner DW, Marfo Y, Hamelmann C, Horstmann RD: Onchocerca volvulus‑exposed persons fail to produce interferon‑gamma in response to O. volvulus antigen but mount proliferative responses with interleukin‑5 and IL‑13 production that decrease with increasing microfilarial density. J Infect Dis. 2002 Apr 15; 185(8):1148‑54. < PMID: 11930325 > | Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA: Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006 Jul; 118(3):353‑60. < PMID: 16827896 > | Kosub DA, Lehrman G, Milush JM, Zhou D, Chacko E, Leone A, Gordon S, Silvestri G, Else JG, Keiser P, Jain MK, Sodora DL: Gamma/Delta T‑Cell Functional Responses Differ after Pathogenic Human Immunodeficiency Virus and Nonpathogenic Simian Immunodeficiency Virus Infections. J Virol. 2008 Feb; 82(3):1155‑1165. < PMID: 18045946 > | Erik L Brincks, Tamara A Kucaba, Kevin L Legge, Thomas S Griffith: Influenza‑induced expression of functional TNF‑related apoptosis‑inducing ligand (TRAIL) on human PBMC. Hum Immunol. 2008 Oct; 69(10):634‑646. < PMID: 18723061 >