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CyFlow™ IgM FITC

CyFlow™ IgM FITC
Antibody: Yes
Antigen: IgM
Application: Flow cytometry
Clonality: monoclonal
Clone: CH2
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunoglobulins
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AA159768

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ IgM FITC
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenPurified human IgM
Background InformationImmunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975; 194:77‑95. < PMID: 47690 > | Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May; 21(6):667‑71. < PMID: 1122610 > | Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr; 39(2):129‑34. < PMID: 6966908 > | Brinkmann V, Heusser CH: T cell‑dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec; 152(2):323‑32. < PMID: 8258141 >

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