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CyFlow™ IgG2b Purified
| Antibody: | Yes |
| Antigen: | IgG2b Isotype Control |
| Application: | Flow cytometry (Isotype control) |
| Clonality: | monoclonal |
| Clone: | PLRV219 |
| Field of Interest: | Isotype controls |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG2b |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Product number: | CK823368 |
For Research Use Only
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ IgG2b Purified
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Not available |
| Background Information | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PLRV219 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Usage | The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10·µg/ml of incubation mixture), change the experimental conditions to reduce the background. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Specific References
| Glier H, Holada K: Blood storage affects the detection of cellular prion protein on peripheral blood leukocytes and circulating dendritic cells in part by promoting platelet satellitism.. J Immunol Methods. 2012 Jun 29; 380(1-2):65-72. < PMID: 22522115 > | Rygh CB, Wang J, Thuen M, Gras Navarro A, Huuse EM, Thorsen F, Poli A, Zimmer J, Haraldseth O, Lie SA, Enger PØ, Chekenya M: Dynamic Contrast Enhanced MRI Detects Early Response to Adoptive NK Cellular Immunotherapy Targeting the NG2 Proteoglycan in a Rat Model of Glioblastoma. PLoS One. 2014 Sep 30; 9(9):e108414. < PMID: 25268630 >