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CyFlow™ IgM Purified

CyFlow™ IgM Purified
Antibody: Yes
Antigen: IgM Isotype Control
Application: Flow cytometry (Isotype control)
Clonality: monoclonal
Clone: PFR-03
Field of Interest: Isotype controls
Format/Fluorochrome: Purified
Isotype: IgM
Regulatory Status: RUO
Source Species: Mouse
Product number: AP063643

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ IgM Purified

Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenNot available
Background InformationThe specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
UsageThe reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10·µg/ml of incubation mixture), change the experimental conditions to reduce the background.
Storage BufferThe reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Michailowsky V, Luhrs K, Rocha MO, Fouts D, Gazzinelli RT, Manning JE: Humoral and cellular immune responses to Trypanosoma cruzi-derived paraflagellar rod proteins in patients with Chagas' disease.. Infect Immun. 2003 Jun; 71(6):3165-71. < PMID: 12761095 >

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