Article successfully added.

CyFlow™ CD2 PE

CyFlow™ CD2 PE
Alternative Name: LFA-2
Antibody: Yes
Antigen: CD2
Application: Flow cytometry
Clonality: monoclonal
Clone: LT2
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CG446271

For Research Use Only

HLDA Workshop HLDA VI—WS Code T 6T-008 Quantity 100 tests Volume 2.0 mL Immunogen... more
CyFlow™ CD2 PE

HLDA WorkshopHLDA VI—WS Code T 6T-008
Quantity100 tests
Volume2.0 mL
ImmunogenNormal human blood lymphocytes
Background InformationCD2 (LFA-2) belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Filatov AV, Bachurin PS, Markova NA, Surnakova NE: A panel of monoclonal antibodies against human lymphocyte antigens. Eksp Onkol. 1989; 11(2):28‑32. < PMID: 2737128 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass‑supported planar bilayers reconstituted with the immunoglobulin‑related adhesion molecule CD58. J Biol Chem. 1997 Jun 20; 272(25):15782‑8. < PMID: 9188475 > | Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta‑Endorphin and natural killer cell cytolytic activity during prolonged exercise: Is there a connection?. Am J Physiol. 1998 Dec; 275(6‑2):R1725‑34. < PMID: 9843861 > | Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15; 106(10):3567‑74. < PMID: 16046525 > | Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of Cellular Avidity Regulation in CD2‑CD58‑Mediated T Cell Adhesion. ACS Chem Biol. 2006 Nov 21; 1(10):649‑58. < PMID: 17168569 > | Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21; 13(31):4207‑13. < PMID: 17696249 >