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CyFlow™ CD3 act. PE

CyFlow™ CD3 act. PE
Antigen: CD3 (activation epitope)
Application: Flow cytometry
Clonality: monoclonal
Clone: APA1/1
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Mouse
Product number: CM152332

For Research Use Only

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HLDA Workshop HLDA III—WS Code T 126 Concentration Unit mg/mL Concentration 0,1... more
CyFlow™ CD3 act. PE
HLDA WorkshopHLDA III—WS Code T 126
Concentration Unitmg/mL
Quantity0.1 mg
Volume1.0 mL
ImmunogenPurified human CD3 proteins isolated from thymus
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Available Technical Data Sheets

Specific References

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