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CyFlow™ CD3 zeta Purified

CyFlow™ CD3 zeta Purified
Antibody: Yes
Antigen: CD3 ζ (Phospho-Tyr72)
Application: Flow cytometry, Immunocytochemistry, Western blot
Clonality: monoclonal
Clone: EM-26
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Mouse
Product number: CK521072

For Research Use Only

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HLDA Workshop HLDA VI—WS Code T 6T-CD3.1 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD3 zeta Purified
HLDA WorkshopHLDA VI—WS Code T 6T-CD3.1
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenA phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 ζ linked to KLH
Background InformationCD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Dopfer EP, Schöpf B, Louis-Dit-Sully C, Dengler E, Höhne K, Klescová A, Prouza M, Suchanek M, Reth M, Schamel WW: Analysis of novel phospho‑ITAM specific antibodies in a S2 reconstitution system for TCR‑CD3 signalling. Immunol Lett. 2010 May 4; 130(1‑2):43‑50. < PMID: 20005895 > | Borroto A, Arellano I, Dopfer EP, Prouza M, Suchanek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1; 190(3):1103‑12. < PMID: 23267019 >