CyFlow™ CD4 Purified
| Alternative Name: | Leu3a, T4 |
| Antibody: | Yes |
| Antigen: | CD4 |
| Application: | Flow cytometry, Immunoprecipitation |
| Clonality: | monoclonal |
| Clone: | MEM-16 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Purified |
| Isotype: | IgM |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CT672597 |
For Research Use Only
| HLDA Workshop | HLDA V—WS Code T T-CD04.10 HLDA VI—WS Code T 6T-013 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Human Acute Lymphocytic Leukemia (ALL) T cells |
| Background Information | CD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. |
| Storage Buffer | The reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17; 198(10):1453‑62. < PMID: 14610046 > | Foster-Cuevas M, Wright GJ, Puklavec MJ, Brown MH, Barclay AN: Human herpesvirus 8 K14 protein mimics CD200 in down‑regulating macrophage activation through CD200 receptor. J Virol. 2004 Jul; 78(14):7667‑76. < PMID: 15220441 > | Clancy L, Mruk K, Archer K, Woelfel M, Mongkolsapaya J, Screaton G, Lenardo MJ, Chan FK: Preligand assembly domain‑mediated ligand‑independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL‑induced apoptosis. Proc Natl Acad Sci USA. 2005 Dec 13; 102(50):18099‑104. < PMID: 16319225 > | Mikesch JH, Buerger H, Simon R, Brandt B: Decay‑accelerating factor (CD55): a versatile acting molecule in human malignancies. Biochim Biophys Acta. 2006 Aug; 1766(1):42‑52. < PMID: 16784816 >