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CyFlow™ CD8a Pacific Blue™

CyFlow™ CD8a Pacific Blue™
Alternative Name: Leu2, T8
Antibody: Yes
Antigen: CD8a
Application: Flow cytometry
Clonality: monoclonal
Clone: 53-6.7
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: Pacific Blue™
Isotype: IgG2a
Laser: Violet
Regulatory Status: RUO
Source Species: Rat
Target Species: Mouse
Product number: BP325091

For Research Use Only

Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
CyFlow™ CD8a Pacific Blue™
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenMouse spleen cells
Background InformationCD8a (CD8 α) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 8·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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