CyFlow™ CD8a Purified
| Alternative Name: | Leu2, T8 |
| Antibody: | Yes |
| Antigen: | CD8a |
| Application: | Flow cytometry, Functional assays, Immunohistochemistry, Immunoprecipitation, Western blot |
| Clonality: | monoclonal |
| Clone: | OX-8 |
| Field of Interest: | Immunophenotyping, MHC |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Rat |
| Product number: | CV104204 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | High Mw glycoproteins from rat thymocytes |
| Background Information | CD8a (CD8 α) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Barclay AN: The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr; 42(4):593‑600. < PMID: 7016746 > | Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov; 22(11):2841‑8. < PMID: 1358623 > | Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15; 158(4):1833‑40. < PMID: 9029123 > | Mitnacht R, Bischof A, Torres-Nagel N, Hünig T: Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J Immunol. 1998 Jan 15; 160(2):700‑7. < PMID: 9551905 > | Ishida S, Usui T, Yamashiro K, Kaji Y, Amano S, Ogura Y, Hida T, Oguchi Y, Ambati J, Miller JW, Gragoudas ES, Ng YS, D'Amore PA, Shima DT, Adamis AP: VEGF164‑mediated inflammation is required for pathological, but not physiological, ischemia‑induced retinal neovascularization. J Exp Med. 2003 Aug 4; 198(3):483‑9. < PMID: 12900522 > | Katsumata Y, Harigai M, Sugiura T, Kawamoto M, Kawaguchi Y, Matsumoto Y, Kohyama K, Soejima M, Kamatani N, Hara M: Attenuation of experimental autoimmune myositis by blocking ICOS‑ICOS ligand interaction. J Immunol. 2007 Sep 15; 179(6):3772‑9. < PMID: 17785814 > | Pino SC, O'Sullivan-Murphy B, Lidstone EA, Yang C, Lipson KL, Jurczyk A, diIorio P, Brehm MA, Mordes JP, Greiner DL, Rossini AA, Bortell R: CHOP mediates endoplasmic reticulum stress‑induced apoptosis in Gimap5‑deficient T cells. PLoS One. 2009; 4(5):e5468. < PMID: 19424493 > | Abe Y, Urakami H, Ostanin D, Zibari G, Hayashida T, Kitagawa Y, Grisham MB: Induction of Foxp3‑expressing regulatory T‑cells by donor blood transfusion is required for tolerance to rat liver allografts. PLoS One. 2009 Nov 23; 4(11):e7840. < PMID: 19956764 >