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CyFlow™ CD31 Purified

CyFlow™ CD31 Purified
Alternative Name: endoCAM, PECA1, PECAM-1
Antibody: Yes
Antigen: CD31
Application: Flow cytometry, Western blot
Clonality: monoclonal
Clone: MEM-05
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BT257374

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD31 Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenLeukocytes of a patient suffering from LGL-type leukemia
Background InformationCD31 (PECAM-1; platelet endothelial cell adhesion molecule-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15; 166(4):2364‑71. < PMID: 11160294 > | Newman DK, Hamilton C, Newman PJ: Inhibition of antigen‑receptor signaling by Platelet Endothelial Cell Adhesion Molecule‑1 (CD31 requires functional ITIMs, SHP‑2, and p56(lck). Blood. 2001 Apr 15; 97(8):2351‑7. < PMID: 11290597 > | Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule‑1 (PECAM‑1/CD31 acts as a regulator of B‑cell development, B‑cell antigen receptor (BCR)‑mediated activation, and autoimmune disease. Blood. 2002 Jul 1; 100(1):184‑9. < PMID: 12759520 > | Jackson DE: The unfolding tale of PECAM‑1. FEBS Lett. 2003 Apr 10; 540(1‑3):41821. < PMID: 12681475 > | Wee JL, Jackson DE: The Ig‑ITIM superfamily member PECAM‑1 regulates the "outside‑in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005 Dec 1; 106(12):3816‑23. < PMID: 16081692 > | Woodfin A, Voisin MB, Nourshargh S: PECAM‑1: a multi‑functional molecule in inflammation and vascular biolog. Arterioscler Thromb Vasc Biol. 2007 Dec; 27(12):2514‑23. < PMID: 17872453 > | Wong MX, Hayball JD, Jackson DE: PECAM‑1‑regulated signalling thresholds control tolerance in anergic transgenic B‑cells. Mol Immunol. 2008 Mar; 45(6):1767‑81. < PMID: 17977600 > | Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti‑CD31 antibodies. PLoS One. 2012; 7(12):e52815. < PMID: 23285192 >