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CyFlow™ CD34 Azide Free

CyFlow™ CD34 Azide Free
Antibody: Yes
Antigen: CD34
Application: Flow cytometry, Functional assays, Immunohistochemistry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: QBEnd-10
Field of Interest: Immunophenotyping
Format/Fluorochrome: Azide Free
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: AH219541

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD34 Azide Free
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman endothelial vesicles
Background InformationCD34 (Mucosialin) is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Schlingemann RO, Rietveld FJ, de Waal RM, Bradley NJ, Skene AI, Davies AJ, Greaves MF, Denekamp J, Ruiter DJ: Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. Lab Invest. 1990 Jun; 62(6):690‑6. < PMID: 1694254 > | Ramani P, Bradley NJ, Fletcher CD: QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections. Histopathology. 1990 Sep; 17(3):237‑42. < PMID: 2242851 > | Kuzu I, Bicknell R, Harris AL, Jones M, Gatter KC, Mason DY: Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol. 1992 Feb; 45(2):143‑8. < PMID: 1371777 > | Sutherland DR, Marsh JC, Davidson J, Baker MA, Keating A, Mellors A: Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34‑positive progenitor cells. Exp Hematol. 1992 Jun; 20(5):590‑9. < PMID: 1375160 > | Grimsley PG, Amos TA, Gordon MY, Greaves MF: Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond. Leukemia. 1993 Jun; 7(6):898‑908. < PMID: 7684800 > | Poblet E, Jimenez-Acosta F, Rocamora A: QBEND/10 (anti‑CD34 antibody) in external root sheath cells and follicular tumors. J Cutan Pathol. 1994 Jun; 21(3):224‑8. < PMID: 7525669 > | Traoré Y, Hirn J: Certain anti‑CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18‑dependent and a CD18‑independent way. Eur J Immunol. 1994 Oct; 24(10):2304‑11. < PMID: 7523134 > | Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor‑mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti‑HPCA‑1. J Hematother Stem Cell Res. 2001 Dec; 10(6):863‑71. < PMID: 11798512 > | Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1; 101(1):112‑8. < PMID: 12589631 > | Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three‑dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk‑L. Stem Cells Dev. 2005 Oct; 14(5):470‑7. < PMID: 16305332 > | Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun; 15(3):305‑13. < PMID: 16846369 >