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CyFlow™ CD35 FITC

CyFlow™ CD35 FITC
Alternative Name: C3b/C4b-R, CR1
Antibody: Yes
Antigen: CD35
Application: Flow cytometry
Clonality: monoclonal
Clone: E11
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: BL832690

For Research Use Only

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HLDA Workshop HLDA III—WS Code 204 Quantity 100 tests Volume 0.4 mL Immunogen... more
CyFlow™ CD35 FITC
HLDA WorkshopHLDA III—WS Code 204
Quantity100 tests
Volume0.4 mL
ImmunogenAcute monocytic leukemia cells and normal blood monocytes
Background InformationCD35 (CR1; complement receptor 1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ: Identification of an anti‑monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar; 14(3):236‑43. < PMID: 6368248 > | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford University Press, Oxford. 1987; 1‑1050. < NLM ID: 8913266 > | Nielsen CH, Pedersen ML, Marquart HV, Prodinger WM, Leslie RG: The role of complement receptors type 1 (CR1, CD35 and 2 (CR2, CD21 in promoting C3 fragment deposition and membrane attack complex formation on normal peripheral human B cells. Eur J Immunol. 2002 May; 32(5):1359‑67. < PMID: 11981823 > | Kremlitzka M, Polgár A, Fülöp L, Kiss E, Poór G, Erdei A: Complement receptor type 1 (CR1, CD35 is a potent inhibitor of B‑cell functions in rheumatoid arthritis patients. Int Immunol. 2013 Jan; 25(1):25‑33. < PMID: 22962438 >