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CyFlow™ CD36 FITC

CyFlow™ CD36 FITC
Alternative Name: GP3B, GPIV
Antibody: Yes
Antigen: CD36
Application: Flow cytometry
Clonality: monoclonal
Clone: CB38 (NL07)
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: FITC
Isotype: IgM
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AJ743875

For Research Use Only

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HLDA Workshop HLDA IV—WS Code P106 Quantity 100 tests Volume 0.4 mL Immunogen... more
CyFlow™ CD36 FITC
HLDA WorkshopHLDA IV—WS Code P106
Quantity100 tests
Volume0.4 mL
ImmunogenLiving human myeloid cells
Background InformationCD36 (FAT; fatty acid translocase) is an 85-113 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F: Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr Stud Hematol Blood Transfus. 1991; 58:182‑6. < PMID: 1720081 > | Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F: Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell Immunol. 1991 Oct 15; 137(2):487‑500. < PMID: 1716523 > | Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F: Platelet activation and inhibition of malarial cytoadherence by the anti‑CD36 IgM monoclonal antibody NL07. Blood. 1993 Dec 15; 82(12):3637‑47. < PMID: 7505121 > | Fadok VA, Warner ML, Bratton DL, Henson PM: CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J Immunol. 1998 Dec 1; 161(11):6250‑7. < PMID: 9834113 > | Matasić R, Dietz AB, Vuk-Pavlović S: Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells. J Leukoc Biol. 1999 Dec; 66(6):909‑14. < PMID: 10614771 > | Kapinsky M, Torzewski M, Büchler C, Duong CQ, Rothe G, Schmitz G: Enzymatically degraded LDL preferentially binds to CD14(high) CD16(+) monocytes and induces foam cell formation mediated only in part by the class B scavenger‑receptor CD36. Arterioscler Thromb Vasc Biol. 2001 Jun; 21(6):1004‑10. < PMID: 11397711 > | McKenna RW, Washington LT, Aquino DB, Picker LJ, Kroft SH: Immunophenotypic analysis of hematogones (B‑lymphocyte precursors) in 662 consecutive bone marrow specimens by 4‑color flow cytometry. Blood. 2001 Oct 15; 98(8):2498‑507. < PMID: 11588048 > | Hoffmann PR, deCathelineau AM, Ogden CA, Leverrier Y, Bratton DL, Daleke DL, Ridley AJ, Fadok VA, Henson PM: Phosphatidylserine (PS) induces PS receptor‑mediated macropinocytosis and promotes clearance of apoptotic cells. J Cell Biol. 2001 Nov 12; 155(4):649‑59. < PMID: 11706053 > | Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 2006 Jun; 13(6):704‑7. < PMID: 16760331 >