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CyFlow™ CD38 Biotin

CyFlow™ CD38 Biotin
Alternative Name: T10
Antibody: Yes
Antigen: CD38
Application: Flow cytometry
Clonality: monoclonal
Clone: HIT2
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BL577767

For Research Use Only

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HLDA Workshop HLDA III—WS Code T 155 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD38 Biotin
HLDA WorkshopHLDA III—WS Code T 155
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman thymocytes in foetus
Background InformationCD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. Another role of CD38 is provided by enzymatic activity of its extracellular part. CD38 acts as NAD+ glycohydrolase converting NAD+ into ADP-ribose, as ADP-ribosyl cyclase producing cADPR and as cADPR hydrolase, thus affecting levels of calcium-mobilizing metabolites. ADPR produced by CD38 serves as an important second messenger of neutrophil and dendritic cell migration.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford University Press, Oxford. 1987; 1‑1050. < NLM ID: 8913266 > | Cakir-Kiefer C, Muller-Steffner H, Oppenheimer N, Schuber F: Kinetic competence of the cADP‑ribose‑CD38 complex as an intermediate in the CD38/NAD+ glycohydrolase‑catalysed reactions: implication for CD38 signalling. Biochem J. 2001 Sep 1; 358(2):399‑406. < PMID: 11513738 > | Lund FE, Muller-Steffner H, Romero-Ramirez H, Moreno-García ME, Partida-Sánchez S, Makris M, Oppenheimer NJ, Santos-Argumedo L, Schuber F: CD38 induces apoptosis of a murine pro‑B leukemic cell line by a tyrosine kinase‑dependent but ADP‑ribosyl cyclase‑ and NAD glycohydrolase‑independent mechanism. Int Immunol. 2006 Jul; 18(7):1029‑42. < PMID: 16720618 > | Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15; 109(6):2545‑52. < PMID: 17132718 > | Partida-Sanchez S, Gasser A, Fliegert R, Siebrands CC, Dammermann W, Shi G, Mousseau BJ, Sumoza-Toledo A, Bhagat H, Walseth TF, Guse AH, Lund FE: Chemotaxis of mouse bone marrow neutrophils and dendritic cells is controlled by adp‑ribose, the major product generated by the CD38 enzymereaction. J Immunol. 2007 Dec 1; 179(11):7827‑39. < PMID: 18025229 > | Rozková D, Novotná L, Pytlík R, Hochová I, Kozák T, Bartůnková J, Spísek R: Toll‑like receptors on B‑CLL cells: expression and functional consequences of their stimulation. Int J Cancer. 2010 Mar 1; 126(5):1132‑43. < PMID: 19685493 >