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CyFlow™ CD44 FITC

CyFlow™ CD44 FITC
Alternative Name: H-CAM , Pgp-1
Antibody: Yes
Antigen: CD44
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-263
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Dog, Human, Pig
Product number: CQ863904

For Research Use Only

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Quantity 100 tests Volume 2.0 mL Immunogen COS-7 African green monkey kidney cells... more
CyFlow™ CD44 FITC
Quantity100 tests
Volume2.0 mL
ImmunogenCOS-7 African green monkey kidney cells
Background InformationCD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford University Press, Oxford. 1987; 1‑1050. < NLM ID: 8913266 > | Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G: Down‑regulation of CD44 contributes to the differentiation of HL‑60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb; 4(1):59‑63. < PMID: 17349212 > | Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA: CD44 regulates macrophage recruitment to the lung in lipopolysaccharide‑induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug; 37(2):248‑53. < PMID: 17446529 > | Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp Mol Pathol. 2007 Oct; 83(2):207‑15. < PMID: 17599831 > | Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec; 83(3):341‑6. < PMID: 17945213 > | Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan‑CD44‑ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12; < PMID: 20811967 >