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CyFlow™ CD45 PerCP

CyFlow™ CD45 PerCP
Alternative Name: B220, LCA , T200
Antibody: Yes
Antigen: CD45
Application: Flow cytometry
Clonality: monoclonal
Clone: EM-05
Emission Maximum: 678 nm
Excitation Maximum: 482 to 490 nm
Field of Interest: Immunophenotyping, MHC, Phosphorylation
Format/Fluorochrome: PerCP
Isotype: IgG
Laser: Blue , Green
Regulatory Status: RUO
Source Species: Rat
Target Species: Mouse
Product number: AP194285

For Research Use Only

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Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
CyFlow™ CD45 PerCP
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenMouse peripheral blood leukocytes
Background InformationCD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 4·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Byth KF, Conroy LA, Howlett S, Smith AJ, May J, Alexander DR, Holmes N: CD45‑null transgenic mice reveal a positive regulatory role for CD45 in earlythymocyte development, in the selection of CD4+CD8+ thymocytes, and B cell maturation. J Exp Med. 1996 Apr 1; 183(4):1707‑18. < PMID: 8666928 > | Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide‑induced microglial activation in mice. Neurosci Lett. 2004 May 13; 362(1):26‑30. < PMID: 15147773 > | Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15; 176(6):3417‑25. < PMID: 16517710 > | Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008 Feb; 13(2):197‑212. < PMID: 18157742 > | Shen KY, Song YC, Chen IH, Chong P, Liu SJ: Depletion of tumor‐associated macrophages enhances the anti‐tumor immunity induced by a Toll‐like receptor agonist‐conjugated peptide. HumVaccinImmunother. 2014; 10(11):3241‐50. < PMID: 25483652 >