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CyFlow™ ARHGEF4 Purified

CyFlow™ ARHGEF4 Purified
Antibody: Yes
Antigen: ARHGEF4
Application: Flow cytometry, Western blot
Clonality: monoclonal
Clone: ARHGEF-08
Field of Interest: Signaling
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CC233224

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ ARHGEF4 Purified
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenRecombinant fragment of human ARHGEF4 (amino acids 143-271)
Background InformationARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF1 (Adenomatous polyposis coli - stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3‑kinase. J Biol Chem. 2009 Aug 14; 284(33):22436‑43. < PMID: 19525225 > | Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL‑AML1 to promote leukaemic transformation. Leuk Res. 2010 Jan; 34(1):109‑15. < PMID: 19628279 > | Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell Res. 2012 Feb; 22(2):372‑86. < PMID: 21788986 >