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CyFlow™ Bcl2 FITC

CyFlow™ Bcl2 FITC
Antibody: Yes
Antigen: Bcl2
Application: Flow cytometry
Clonality: monoclonal
Clone: Bcl-2/100
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Apoptosis
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AY147314

For Research Use Only

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Quantity 100 tests Volume 0.4 mL Immunogen Synthetic peptide corresponding to the... more
CyFlow™ Bcl2 FITC
Quantity100 tests
Volume0.4 mL
ImmunogenSynthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Background InformationBcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Soilu-Hänninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl‑2‑independent pathway. J Neurosci. 1999 Jun 15; 19(12):4828‑38. < PMID: 10366617 > | Laflamme C, Israël-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb; 21(2):225‑31. < PMID: 12608434 > | Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl‑2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul; 13(7):1235‑7. < PMID: 16485035 > | Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef. J Virol. 2007 Oct; 81(20):11426‑40. < PMID: 17670831 > | Joubert A, Marais S, Maritz C: Influence of 2‑methoxyestradiol on MCF‑7 cells: an improved differential interference contrasting technique and Bcl‑2 and Bax protein expression levels. Biocell. 2009 Apr; 33(1):67‑70. < PMID: 19499887 >