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CyFlow™ Beta2-MG Alexa Fluor™ 647

CyFlow™ Beta2-MG Alexa Fluor™ 647
Antibody: Yes
Antigen: β2-microglobulin
Application: Flow cytometry
Clonality: monoclonal
Clone: B2M-01
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Field of Interest: Blood group antigens, MHC
Format/Fluorochrome: Alexa Fluor™ 647
Isotype: IgG2a
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AB793544

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ Beta2-MG Alexa Fluor™ 647
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenPurified human β2-microglobulin
Background Informationβ2-microglobulin (β2M) non-covalently associates with the 44 kDa α chain to forms the HLA Class I antigen complex. Human β2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M‑01 for detection and purification of human beta 2‑microglobulin. Folia Biol (Praha). 1984; 30(6):369‑76. < PMID: 6394369 > | Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA‑G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24):15226‑15237. < PMID: 16306594 > | Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep; 21(9):1257‑65. < PMID: 16810512 > | Poláková K, Železníková T, Russ G: HLA‑G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb; 37(2):139‑45. < PMID: 23146557 >