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CyFlow™ Beta-catenin Purified

CyFlow™ Beta-catenin Purified
Antibody: Yes
Antigen: β-catenin
Application: Flow cytometry, Immunocytochemistry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: EM-22
Field of Interest: Adaptor proteins, Cytoskeleton, Transcription
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Hamster, Human, Mouse
Product number: BP858553

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ Beta-catenin Purified
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenRecombinant human β-catenin
Background Informationβ-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound α, β and γ catenin. β-catenin links this complex through α-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumor cells. β-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 β. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of β-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Valenta T, Lukas J, Korinek V: HMG box transcription factor TCF‑4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1; 31(9):2369‑80. < PMID: 12711682 > | Nowak M, Madej JA, Dziegiel P: Expression of E‑cadherin, beta‑catenin and Ki‑67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia Histochem Cytobiol. 2007; 45(3):233‑8. < PMID: 17951173 > | Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS: Epstein‑Barr Virus, Beta‑Catenin, and E‑cadherin in Gastric Carcinomas. J Korean Med Sci. 2007 Oct; 22(5):855‑61. < PMID: 17982235 >