CyFlow™ CD41 Biotin
| Alternative Name: | gpIIb |
| Antibody: | Yes |
| Antigen: | CD41 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-06 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BS343972 |
For Research Use Only
| HLDA Workshop | HCDM/HLDA 10 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Leukocytes of a patient suffering from LGL-type leukemia |
| Background Information | CD41 (GPIIb; platelet glycoprotein IIb) is composed of two subunits (120 kDa GPIIb α and 23 kDa GPIIb β) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Debili N, Robin C, Schiavon V, Letestu R, Pflumio F, Mitjavila-Garcia MT, Coulombel L, Vainchenker W: Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates. Blood. 2001 Apr 1; 97(7):2023‑30. < PMID: 11264167 > | Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W: Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development. 2002 Apr; 129(8):2003‑13. < PMID: 11934866 > | Ferkowicz MJ, Starr M, Xie X, Li W, Johnson SA, Shelley WC, Morrison PR, Yoder MC: CD41 expression defines the onset of primitive and definitive hematopoiesis inthe murine embryo. Development. 2003 Sep; 130(18):4393‑403. < PMID: 12900455 > | Zhang J, Varas F, Stadtfeld M, Heck S, Faust N, Graf T: CD41‑YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation. Exp Hematol. 2007 Mar; 35(3):490‑499. < PMID: 17309829 >