CyFlow™ CD42a Purified
| Alternative Name: | GPIX |
| Antibody: | Yes |
| Antigen: | CD42a |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | GR-P |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Dog, Human |
| Product number: | AK114812 |
For Research Use Only
| HLDA Workshop | HLDA IV—WS Code P 35 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Human Acute Lymphocytic Leukemia (ALL) T cells |
| Background Information | CD42a (GPIX; Glycoprotein 9) composes together with GPIb α, GPIb β and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. Defects in the gene encoding CD42a are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Brown SB, Clarke MC, Magowan L, Sanderson H, Savill J: Constitutive death of platelets leading to scavenger receptor‑mediated phagocytosis: A caspase‑independent cell clearance program. J Biol Chem. 2000 Feb 25; 275(8):5987‑96. < PMID: 10681593 > | Harding SA, Din JN, Sarma J, Josephs DH, Fox KA, Newby DE: Promotion of proinflammatory interactions between platelets and monocytes by unfractionated heparin. Heart. 2006 Nov; 92(11):1635‑8. < PMID: 16709700 > | Vettore S, Scandellari R, Moro S, Lombardi AM, Scapin M, Randi ML, Fabris F: Novel point mutation in a leucine‑rich repeat of the GPIb alpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard‑Soulier syndrome affecting two unrelated families: the N41H variant. Haematologica. 2008 Nov; 93(11):1743‑7. < PMID: 18815197 > | Din JN, Aftab SM, Jubb AW, Carnegy FH, Lyall K, Sarma J, Newby DE, Flapan AD: Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans. Eur J Clin Nutr. 2011 Feb; 65(2):234‑9. < PMID: 21048773 >