CyFlow™ CD54 Biotin
| Alternative Name: | ICAM-1 |
| Antibody: | Yes |
| Antigen: | CD54 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | 1H4 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG2b |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CH673649 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Raji cells and spleen cells fused with NS1 cells |
| Background Information | CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM‑1) has a central role in cell‑cell contact‑mediated immune mechanisms. Proc Natl Acad Sci USA. 1988 May; 85(9):3095‑9. < PMID: 3362863 > | Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM‑1) during lymphohematopoietic differentiation. Blood. 1989 May 15; 73(7):1896‑903. < PMID: 2469503 > | Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2; 346(6283):425‑34. < PMID: 1974032 > | Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum‑infected erythrocytes bind ICAM‑1 at a site distinct from LFA‑1, Mac‑1, and human rhinovirus. Cell. 1992 Jan 10; 68(1):63‑9. < PMID: 1346257 > | Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay‑accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J Gen Virol. 2004 Mar; 85(3):731‑8. < PMID: 14993659 >