CyFlow™ CD66e Purified
| Alternative Name: | CEA |
| Antibody: | Yes |
| Antigen: | CD66e |
| Application: | Flow cytometry, Immunohistochemistry, Immunoprecipitation |
| Clonality: | monoclonal |
| Clone: | CB30 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CU348583 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Human carcinoembryonic antigen (CEA; CEACAM5) |
| Background Information | CD66e (CEA) is a 180-200 kDa member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Oikawa S, Nakazato H, Kosaki G: Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence. Biochem Biophys Res Commun. 1987 Jan 30; 142(2):511‑8. < PMID: 3814146 > | Thompson JA, Grunert F, Zimmermann W: Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal. 1991; 5(5):344‑66. < PMID: 1941355 > | Chen CJ, Li LJ, Maruya A, Shively JE: In vitro and in vivo footprint analysis of the promoter of carcinoembryonic antigen in colon carcinoma cells: effects of interferon gamma treatment. Cancer Res. 1995 Sep 1; 55(17):3873‑82. < PMID: 7641207 > | Gangopadhyay A, Bajenova O, Kelly TM, Thomas P: Carcinoembryonic antigen induces cytokine expression in Kuppfer cells: implications for hepatic metastasis from colorectal cancer. Cancer Res. 1996 Oct 15; 56(20):4805‑10. < PMID: 8841002 > | Koga S, Oshima Y, Honkura N, Iimura T, Kameda K, Sato K, Yoshida M, Yamamoto Y, Watanabe Y, Hikita A, Imamura T: In vivo subcellular imaging of tumors in mouse models using a fluorophore‐conjugated anti‐carcinoembryonic antigen antibody in two‐photon excitation microscopy. CancerSci. 2014 Oct; 105(10):1299‐306. < PMID: 25117702 > | Wang L, Ma N, Okamoto S, Amaishi Y, Sato E, Seo N, Mineno J, Takesako K, Kato T, Shiku H: Efficient tumor regression by adoptively transferred CEA‐specific CAR‐T cells associated with symptoms of mild cytokine release syndrome. Oncoimmunology. 2016 Jul 25; 5(9):e1211218. < PMID: 27757303 >