Article successfully added.

CyFlow™ CD103 Purified

CyFlow™ CD103 Purified
Alternative Name: HML-1 , Integrin αE-subunit
Antibody: Yes
Antigen: CD103
Application: Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: Ber-ACT8
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: BK166852

For Research Use Only

HLDA Workshop HLDA V—WS Code A067 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD103 Purified
HLDA WorkshopHLDA V—WS Code A067
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHTLV 1-induced human T cell line MAPS16
Background InformationCD103 (Integrin αE) is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-β stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin β7 subunit to form αE/β7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| French JJ, Cresswell J, Wong WK, Seymour K, Charnley RM, Kirby JA: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E‑cadherin in immunotherapy?. Br J Cancer. 2002 Oct 21; 87(9):1034‑41. < PMID: 12434297 > | Poles MA, Barsoum S, Yu W, Yu J, Sun P, Daly J, He T, Mehandru S, Talal A, Markowitz M, Hurley A, Ho D, Zhang L: Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy. J Virol. 2003 Oct; 77(19):10456‑67. < PMID: 12970431 > | Brandes M, Willimann K, Lang AB, Nam KH, Jin C, Brenner MB, Morita CT, Moser B: Flexible migration program regulates gamma delta T‑cell involvement in humoral immunity. Blood. 2003 Nov 15; 102(10):3693‑701. < PMID: 12881309 > | León F, Roy G: Isolation of human small bowel intraepithelial lymphocytes by annexin V‑coated magnetic beads. Lab Invest. 2004 Jun; 84(6):804‑9. < PMID: 15154017 > | Sigmundsdottir H, Johnston A, Gudjonsson JE, Bjarnason B, Valdimarsson H: Methotrexate markedly reduces the expression of vascular E‑selectin, cutaneous lymphocyte‑associated antigen and the numbers of mononuclear leucocytes in psoriatic skin. Exp Dermatol. 2004 Jul; 13(7):426‑34. < PMID: 15217363 > | Cardoso CR, Garlet GP, Moreira AP, Júnior WM, Rossi MA, Silva JS: Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis. J Leukoc Biol. 2008 Jul; 84(1):311‑8. < PMID: 18451325 > | Jaensson E, Uronen-Hansson H, Pabst O, Eksteen B, Tian J, Coombes JL, Berg PL, Davidsson T, Powrie F, Johansson-Lindbom B, Agace WW: Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J Exp Med. 2008 Sep 1; 205(9):2139‑49. < PMID: 18710932 > | Kost CB, Holden JT, Mann KP: Marginal zone B‑cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep; 74(5):282‑6. < PMID: 18500740 > | Kost CB, Holden JT, Mann KP: Marginal zone B‑cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep; 74(5):282‑6. < PMID: 18500740 > | Zhang L, Bertucci AM, Ramsey-Goldman R, Burt RK, Datta SK: Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF‑beta‑producing CD8+ Treg cells are associated with immunological remission of lupus. J Immunol. 2009 Nov 15; 183(10):6346‑58. < PMID: 19841178 >