CyFlow™ CD140a Biotin
Alternative Name: | PDGFRA , PDGRF2 |
Antibody: | Yes |
Antigen: | CD140a |
Application: | Flow cytometry |
Clonality: | monoclonal |
Clone: | 16A1 |
Field of Interest: | Immunophenotyping, Signaling |
Format/Fluorochrome: | Biotin |
Isotype: | IgG1 |
Regulatory Status: | RUO |
Source Species: | Mouse |
Target Species: | Human |
Product number: | AS196282 |
For Research Use Only
Concentration Unit | mg/mL |
Concentration | 1 |
Quantity | 0.1 mg |
Volume | 0.1 mL |
Immunogen | CD140a-transfected NIH 3T3 cells |
Background Information | CD140a (PDGF-RA; platelet-derived growth factor receptor α) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b (PDGF-RB). Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions. |
Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
| Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL‑2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep; 78(3):725‑35. < PMID: 16000396 > | Andrae J, Gallini R, Betsholtz C: Role of platelet‑derived growth factors in physiology and medicine. Genes Dev. 2008 May 15; 22(10):1276‑312. < PMID: 18483217 > | French WJ, Creemers EE, Tallquist MD: Platelet‑derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep; 28(18):5646‑57. < PMID: 18606782 > | Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid‑producing lineages. Genes Dev. 2008 Dec 1; 22(23):3255‑67. < PMID: 19056881 > | Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet‑derived growth factor‑receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug; 11(8):732‑42. < PMID: 19649203 >