CyFlow™ CD161 FITC
| Alternative Name: | NKR, NKR-P1A , NKRP1A |
| Antibody: | Yes |
| Antigen: | CD161 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | 10/78 |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Field of Interest: | Immunophenotyping, MHC |
| Format/Fluorochrome: | FITC |
| Isotype: | IgG1 |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Rat |
| Product number: | CF896142 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 0,5 |
| Quantity | 0.1 mg |
| Volume | 0.2 mL |
| Immunogen | Splenic cells purified from the LEW rat |
| Background Information | CD161 (Nkrp1; natural killer receptor protein 1 or Klrb1; killer cell lectin-like receptor subfamily b member 1) is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a. |
| Usage | The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Sedgwick JD, Ford AL, Foulcher E, Airriess R: Central nervous system microglial cell activation and proliferation follows direct interaction with tissue‑infiltrating T cell blasts. J Immunol. 1998 Jun 1; 160(11):5320‑30. < PMID: 9605131 > | Tliba O, Chauvin A, Le Vern Y, Boulard C, Sbille P: Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats. Vet Res. 2002 May-Jun; 33(3):327‑32. < PMID: 12056483 > | May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD: CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA‑B27 transgenic rats. J Immunol. 2003 Jan 15; 170(2):1099‑105. < PMID: 12517979 > | Stephens LA, Barclay AN, Mason D: Phenotypic characterization of regulatory CD4+CD25+ T cells in rats. Int Immunol. 2004 Feb; 16(2):365‑75. < PMID: 14734622 > | Chang CJ, Tai KF, Roffler S, Hwang LH: The immunization site of cytokine‑secreting tumor cell vaccines influences the trafficking of tumor‑specific T lymphocytes and antitumor efficacy against regional tumors. J Immunol. 2004 Nov 15; 173(10):6025‑32. < PMID: 15528337 > | Teshima R, Nakamura R, Nakamura R, Hachisuka A, Sawada JI, Shibutani M: Effects of exposure to decabromodiphenyl ether on the development of the immune system in rats. J Health Sci. 2008; 54(4):382‑389. < No PMID > | Kraszula L, Eusebio M, Kupczyk M, Kuna P, Pietruczuk M: The use of multi‑color flow cytometry for identification of functional markers of nTregs in patients with severe asthma. Pneumonol Alergol Pol. 2012; 80(5):389‑401. < PMID: 22926900 >