CyFlow™ CD203c PE
| Alternative Name: | B10, E-NPP3 , NPP3, PD-1b, PDNP3 |
| Antibody: | Yes |
| Antigen: | CD203c |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | NP4D6 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PE |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CC495549 |
For Research Use Only
| HLDA Workshop | HLDA VIII |
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | HEK-293 cells transfected with human CD203c |
| Background Information | CD203c (ENPP-3) is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Bühring HJ, Simmons PJ, Pudney M, Müller R, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P, Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood. 1999 Oct 1; 94(7):2343‑56. < PMID: 10498606 > | Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K: The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 31. Blood. 2001 May 15; 97(10):3303‑5. < PMID: 11342463 > | Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring HJ: Hymenoptera‑venom‑induced upregulation of the basophil activation marker ecto‑nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol. 2001 Dec; 126(4):335‑42. < PMID: 11815741 > | Hauswirth AW, Escribano L, Prados A, Nuñez R, Mirkina I, Kneidinger M, Florian S, Sonneck K, Vales A, Schernthaner GH, Sanchez-Muñoz L, Sperr WR, Bühring HJ, Orfao A, Valent P: CD203c is overexpressed on neoplastic mast cells in systemic mastocytosis and is upregulated upon IgE receptor cross‑linking. Int J Immunopathol Pharmacol. 2008 Oct-Dec; 21(4):797‑806. < PMID: 19144265 > | Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG: Allergen‑specific basophil suppression associated with clinical tolerance i patients with milk allergy. J Allergy Clin Immunol. 2009 Apr; 123(4):789‑94e20. < PMID: 19348919 > | Tokuda R, Nagao M, Hiraguchi Y, Hosoki K, Matsuda T, Kouno K, Morita E, Fujisawa T: Antigen‑induced expression of CD203c on basophils predicts IgE‑mediated wheat allergy. Allergol Int. 2009 Jun; 58(2):193‑9. < PMID: 19240377 > | Chirumbolo S, Brizzi M, Ortolani R, Vella A, Bellavite P: Inhibition of CD203c membrane up‑regulation in human basophils by high dilution of histamine: a controlled replication study. Inflamm Res. 2009 Nov; 58(11):755‑64. < PMID: 19418203 >