CyFlow™ CD229 APC
| Alternative Name: | hly9, Ly-9 , mLY9, SLAMF3 |
| Antibody: | Yes |
| Antigen: | CD229 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | HLy9.25 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CH984515 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | CD299-transfected 300.19 pre-B cell line |
| Background Information | CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| de la Fuente MA, Tovar V, Villamor N, Zapater N, Pizcueta P, Campo E, Bosch J, Engel P: Molecular characterization and expression of a novel human leukocyte cell‑surface marker homologous to mouse Ly‑9. Blood. 2001 Jun 1; 97(11):3513‑20. < PMID: 11369645 > | Del Valle JM, Engel P, Martín M: The cell surface expression of SAP‑binding receptor CD229 is regulated via its interaction with clathrin‑associated adaptor complex 2 (AP‑2). J Biol Chem. 2003 May 9; 278(19):17430‑7. < PMID: 12621057 > | Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT‑2‑binding leukocyte cell‑surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens. 2004 Aug; 64(2):132‑44. < PMID: 15245368 > | Martín M, Del Valle JM, Saborit I, Engel P: Identification of Grb2 as a novel binding partner of the signaling lymphocytic activation molecule‑associated protein binding receptor CD229. J Immunol. 2005 May 15; 174(10):5977‑86. < PMID: 15879090 > | Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly{09} lymphocyte cell surface receptor interacts homophilically through its N‑terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1; 174(11):7033‑42. < PMID: 15905546 > | Bund D, Mayr C, Kofler DM, Hallek M, Wendtner CM: Human Ly9 (CD229 as novel tumor‑associated antigen (TAA) in chronic lymphocytic leukemia (B‑CLL) recognized by autologous CD8+ T cells. Exp Hematol. 2006 Jul; 34(7):860‑9. < PMID: 16797413 > | Sintes J, Romero X, Marin P, Terhorst C, Engel P: Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells. Exp Hematol. 2008 Sep; 36(9):1199‑204. < PMID: 18495325 >