CyFlow™ BrdU PE
| Antibody: | Yes |
| Antigen: | 5-bromodeoxyuridine |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | Bu20a |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Small organic molecules |
| Format/Fluorochrome: | PE |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Product number: | CL205376 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | 5-bromodeoxyuridine conjugated to BSA |
| Background Information | 5-bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY: Double immunocytochemical labeling of cell and tissue samples with monoclonal anti‑bromodeoxyuridine. J Histochem Cytochem. 1989 Oct; 37(10):1517‑27. < PMID: 2476478 > | Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB: Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki‑67(MIB‑5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem. 2003 Dec; 51(12):1681‑8. < PMID: 14623936 > | Martín CA, Surur JM, García MN, Corrons F, Badrán AF: Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver. Biocell. 2005 Apr; 29(1):33‑8. < PMID: 15954465 > | Shih CH, Whalen BA, Goto Y, Hogg JC, van Eeden SF: Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'‑bromo‑2'‑deoxyuridine. Am J Physiol Cell Physiol. 2005 Sep; 289(3):C757‑65. < PMID: 15857903 > | Pajalunga D, Mazzola A, Salzano AM, Biferi MG, De Luca G, Crescenzi M: Critical requirement for cell cycle inhibitors in sustaining nonproliferative states. J Cell Biol. 2007 Mar 12; 176(6):807‑18. < PMID: 17353358 > | Lagares A, Li HY, Zhou XF, Avendaňo C: Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio‑neuronal precursor maturation. J Neurosci. 2007 Jul 25; 27(30):7939‑53. < PMID: 17652585 > | Yatera K, Hsieh J, Hogg JC, Tranfield E, Suzuki H, Shih CH, Behzad AR, Vincent R, van Eeden SF: Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques. Am J Physiol Heart Circ Physiol. 2008 Feb; 294(2):H944‑53. < PMID: 18083905 > | O'Driscoll M, Jeggo PA: CsA can induce DNA double‑strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair. Bone Marrow Transplant. 2008 Jun; 41(11):983‑9. < PMID: 18278071 >