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CyFlow™ BrdU Purified

CyFlow™ BrdU Purified
Antibody: Yes
Antigen: 5-bromodeoxyuridine
Application: Flow cytometry, Immunocytochemistry, Immunohistochmistry (paraffin-embedded sections)
Clonality: monoclonal
Clone: MoBu-1
Field of Interest: Small organic molecules, Transcription
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Product number: BQ947443

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ BrdU Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
Immunogen5-bromodeoxyuridine conjugated with hemocyanine
Background Information5-bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Soames AR, Lavender D, Foster JR, Williams SM, Wheeldon EB: Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S‑phase in rat and mouse liver. J Histochem Cytochem. 1994 Jul; 42(7):939‑44. < PMID: 8014477 > | Buckiova D, Kubinova L, Soukup A, Jelinek R, Brown NA: Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects. Int J Dev Biol. 1998 Jul; 42(5):737‑40. < PMID: 9712529 > | Ashby J, Tinwell H, Soames A, Foster J: Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. Environ Health Perspect. 1999 Oct; 107(10):819‑22. < PMID: 10504149 > | Stanek D, Kiss T, Raska I: Pre‑ribosomal RNA is processed in permeabilised cells at the site of transcription. Eur J Cell Biol. 2000 Mar; 79(3):202‑7. < PMID: 10777112 >

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