CyFlow™ Drebrin Purified
| Antibody: | Yes |
| Antigen: | Drebrin |
| Application: | Flow cytometry, Western blot |
| Clonality: | monoclonal |
| Clone: | DBN-N-03 |
| Field of Interest: | Cytoskeleton, Neurobiology |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG2b |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AP230517 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326) |
| Background Information | Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse. |
| Storage Buffer | The reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Ishikawa R, Hayashi K, Shirao T, Xue Y, Takagi T, Sasaki Y, Kohama K: Drebrin, a development‑associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J Biol Chem. 1994 Nov 25; 269(47):29928‑33. < PMID: 7961990 > | Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K: Inhibition by drebrin of the actin‑bundling activity of brain fascin, a protein localized in filopodia of growth cones. J Neurochem. 1996 Mar; 66(3):980‑8. < PMID: 8769857 > | Hayashi K, Ishikawa R, Ye LH, He XL, Takata K, Kohama K, Shirao T: Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex. J Neurosci. 1996 Nov 15; 16(22):7161‑70. < PMID: 8929425 > | Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T: Domain analysis of the actin‑binding and actin‑remodeling activities of drebrin. Exp Cell Res. 1999 Dec 15; 253(2):673‑80. < PMID: 10585290 > | Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F: F‑actin‑binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci. 2010 Apr 1; 123(7):1160‑70. < PMID: 20215400 > | Vaskova M, Kovac M, Volna P, Angelisova P, Mejstrikova E, Zuna J, Brdicka T, Hrusak O: High expression of cytoskeletal protein drebrin in TEL/AML1pos B‑cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody. Leuk Res. 2011 Aug; 35(8):1111‑3. < PMID: 21497902 >