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CyFlow™ FoxP3 Alexa Fluor™ 647

CyFlow™ FoxP3 Alexa Fluor™ 647
Alternative Name: foxp3 ,Forkhead box P3 ,IPEX ,JM2 ,MGC141961 ,MGC141963 ,PIDX ,XPID
Antibody: Yes
Antigen: FoxP3
Application: Flow cytometry
Clonality: monoclonal
Clone: 3G3
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Field of Interest: Transcription
Format/Fluorochrome: Alexa Fluor™ 647
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Mouse
Product number: CA372958

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
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CyFlow™ FoxP3 Alexa Fluor™ 647
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenFull-length His-tagged recombinant murine FoxP3
Background InformationFoxP3 (Forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 6·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single‑cell analysis of normal and FOXP3‑mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci USA. 2006 Apr 25; 103(17):6659‑64. < PMID: 16617117 > | Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 2008; 94:150‑7. < PMID: 18802345 > | Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan; 301(1):71‑81. < PMID: 18985367 > | Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 18; 31(3):401‑11. < PMID: 19766083 > | Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29; 114(18):3727‑35. < PMID: 19641188 > | Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec; 21(6):612‑8. < PMID: 19854631 > | Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec; 75(12):1040‑50. < PMID: 19845018 >

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