CyFlow™ HLA-G Purified
| Antibody: | Yes |
| Antigen: | HLA-G |
| Application: | Enzyme-linked immunosorbent assay, Flow cytometry, Immunoprecipitation |
| Clonality: | monoclonal |
| Clone: | G233 |
| Field of Interest: | MHC |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG2a |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AY912752 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | HLA-A2.1/human β2-microglobulin double transgenic mice were immunized with mouse L cells transfected with both human β2-microglobulin and HLA-G |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Loke YW, King A, Burrows T, Gardner L, Bowen M, Hiby S, Howlett S, Holmes N, Jacobs D: Evaluation of trophoblast HLA‑G antigen with a specific monoclonal antibody. Tissue Antigens. 1997 Aug; 50(2):135‑46. < PMID: 9271823 > | Mandelboim O, Pazmany L, Davis DM, Vales-Gomez M, Reyburn HT, Rybalov B, Strominger JL: Multiple receptors for HLA‑G on human natural killer cells. Proc Natl Acad Sci USA. 1997 Dec 23; 94(26):14666‑70. < PMID: 9405670 > | Rajagopalan S, Long EO: A human histocompatibility leukocyte antigen (HLA)‑G‑specific receptor expressed on all natural killer cells. J Exp Med. 1999 Apr 5; 189(7):1093‑100. < PMID: 10190900 > | Frumento G, Franchello S, Palmisano GL, Nicotra MR, Giacomini P, Loke YW, Geraghty DE, Maio M, Manzo C, Natali PG, Ferrara GB: Melanomas and melanoma cell lines do not express HLA‑G, and the expression cannot be induced by gammaIFN treatment. Tissue Antigens. 2000 Jul; 56(1):42581. < PMID: 10958353 > | Blaschitz A, Hutter H, Dohr G: HLA Class I protein expression in the human placenta. Early Pregnancy. 2001 Jan; 5(1):67‑9. < PMID: 11753519 > | Van Lierop MJ, Wijnands F, Loke YW, Emmer PM, Lukassen HG, Braat DD, van der Meer A, Mosselman S, Joosten I: Detection of HLA‑G by a specific sandwich ELISA using monoclonal antibodies G233 and 56B. Mol Hum Reprod. 2002 Aug; 8(8):776‑84. < PMID: 12149411 > | Bulmer JN, Cioni R, Bussani C, Cirigliano V, Sole F, Costa C, Garcia P, Adinolfi M: HLA‑G positive trophoblastic cells in transcervical samples and their isolation and analysis by laser microdissection and QF‑PCR. Prenat Diagn. 2003 Jan; 23(1):34‑9. < PMID: 12533810 > | Imudia AN, Suzuki Y, Kilburn BA, Yelian FD, Diamond MP, Romero R, Armant DR: Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study. Hum Reprod. 2009 Sep; 24(9):2086‑92. < PMID: 19497946 > | Gonzalez A, Alegre E, Arroyo A, LeMaoult J, Echeveste JI: Identification of circulating nonclassic human leukocyte antigen G (HLA‑G)‑like molecules in exudates. Clin Chem. 2011 Jul; 57(7):1013‑22. < PMID: 21527645 >