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CyFlow™ IgE FITC

CyFlow™ IgE FITC
Antibody: Yes
Antigen: IgE
Application: Flow cytometry
Clonality: monoclonal
Clone: 4H10
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunoglobulins
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BD615264

For Research Use Only

Description Documentation TDS & SDS
Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ IgE FITC
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenPurified human IgE
Background InformationImmunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 3.5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975; 194:77‑95. < PMID: 47690 > | Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May; 21(6):667‑71. < PMID: 1122610 > | Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr; 39(2):129‑34. < PMID: 6966908 > | Brinkmann V, Heusser CH: T cell‑dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec; 152(2):323‑32. < PMID: 8258141 > | Gould HJ, Beavil RL, Vercelli D: IgE isotype determination: epsilon‑germline gene transcription, DNA recombination and B‑cell differentiation. Br Med Bull. 2000; 56(4):908‑24. < PMID: 11359628 >

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