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CyFlow™ IgG (Fab) PE-Cy5

CyFlow™ IgG (Fab) PE-Cy5
Antibody: Yes
Antigen: IgG (Fab)
Application: Flow cytometry
Clonality: monoclonal
Clone: 4A11
Emission Maximum: 670 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunoglobulins
Format/Fluorochrome: PE-Cy5
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BA864768

For Research Use Only

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Purified human IgG Background... more
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CyFlow™ IgG (Fab) PE-Cy5
Quantity100 tests
Volume0.4 mL
ImmunogenPurified human IgG
Background InformationImmunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975; 194:77‑95. < PMID: 47690 > | Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May; 21(6):667‑71. < PMID: 1122610 > | Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr; 39(2):129‑34. < PMID: 6966908 > | Brinkmann V, Heusser CH: T cell‑dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec; 152(2):323‑32. < PMID: 8258141 > | Wang H, Lee HK, Bukowski JF, Li H, Mariuzza RA, Chen ZW, Nam KH, Morita CT: Conservation of nonpeptide antigen recognition by rhesus monkey V gamma 2V delta 2 T cells.. J Immunol. 2003 Apr 1; 170(7):3696-706. < PMID: 12646635 > | Fujii Y, Kaneko S, Nzou SM, Mwau M, Njenga SM, Tanigawa C, Kimotho J, Mwangi AW, Kiche I, Matsumoto S, Niki M, Osada-Oka M, Ichinose Y, Inoue M, Itoh M, Tachibana H, Ishii K, Tsuboi T, Yoshida LM, Mondal D, Haque R, Hamano S, Changoma M, Hoshi T, Kamo K, Karama M, Miura M, Hirayama K: Serological Surveillance Development for Tropical Infectious Diseases Using Simultaneous Microsphere-Based Multiplex Assays and Finite Mixture Models. PLoS Negl Trop Dis. 2014 Jul 31; 8(7):e3040. < PMID: 25078404 >

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