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CyFlow™ Ki-67 Alexa Fluor™ 647

CyFlow™ Ki-67 Alexa Fluor™ 647
Antibody: Yes
Antigen: Ki-67
Application: Flow cytometry
Clonality: monoclonal
Clone: Ki-67
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Field of Interest: Transcription
Format/Fluorochrome: Alexa Fluor™ 647
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Cow, Human
Product number: AD268830

For Research Use Only

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Nuclei of the Hodgkin lymphoma cell line... more
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CyFlow™ Ki-67 Alexa Fluor™ 647
Quantity100 tests
Volume0.4 mL
ImmunogenNuclei of the Hodgkin lymphoma cell line L428
Background InformationKi-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15; 31(1):13‑20. < PMID: 6339421 > | Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation‑associated human nuclear antigen defined by the monoclonal antibody Ki‑67. J Immunol. 1984 Oct; 133(4):1710‑5. < PMID: 6206131 > | Gerdes J: Ki‑67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun; 1(3):199‑206. < PMID: 2103495 > | Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation‑associated antigen of antibody Ki‑67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle‑maintaining proteins. J Cell Biol. 1993 Nov; 123(3):513‑22. < PMID: 8227122 > | Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation‑associated nuclear antigen defined by antibody Ki‑67: a new kind of cell‑cycle‑maintaining proteins. Arch Immunol Ther Exp (Warsz). 1995; 43(2):117‑21. < PMID: 8744726 >

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