CyFlow™ Notch 1 Purified
| Antibody: | Yes |
| Antigen: | Notch 1 |
| Application: | Flow cytometry, Immunocytochemistry, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot |
| Clonality: | monoclonal |
| Clone: | mN1A |
| Field of Interest: | Transcription |
| Format/Fluorochrome: | Purified |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human, Mouse |
| Product number: | AJ233200 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | GST fusion protein containing cdc10-NCR region of mouse Notch 1 |
| Background Information | Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R: Embryonic lethality in mice homozygous for a processing‑deficient allele of Notch1. Nature. 2000 Jun 22; 405(6789):966‑70. < PMID: 10879540 > | Espinosa L, Santos S, Inglés-Esteve J, Muñoz-Canoves P, Bigas A: p65‑NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N‑CoR. J Cell Sci. 2002 Mar 15; 115(6):1295‑303. < PMID: 11884528 > | Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21; 177(4):647‑57. < PMID: 17502421 > | Tagami S, Okochi M, Yanagida K, Ikuta A, Fukumori A, Matsumoto N, Ishizuka-Katsura Y, Nakayama T, Itoh N, Jiang J, Nishitomi K, Kamino K, Morihara T, Hashimoto R, Tanaka T, Kudo T, Chiba S, Takeda M: Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch‑1. Mol Cell Biol. 2008 Jan; 28(1):165‑76. < PMID: 17967888 > | Watanabe K, Nagaoka T, Lee JM, Bianco C, Gonzales M, Castro NP, Rangel MC, Sakamoto K, Sun Y, Callahan R, Salomon DS: Enhancement of Notch receptor maturation and signaling sensitivity by Cripto‑1. J Cell Biol. 2009 Nov 2; 187(3):343‑53. < PMID: 19948478 > | Kefas B, Comeau L, Floyd DH, Seleverstov O, Godlewski J, Schmittgen T, Jiang J, diPierro CG, Li Y, Chiocca EA, Lee J, Fine H, Abounader R, Lawler S, Purow B: The neuronal microRNA miR‑326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J Neurosci. 2009 Dec 2; 29(48):15161‑8. < PMID: 19955368 > | Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV‑1 Rev‑binding protein accelerates cellular uptake of iron to drive Notch‑induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1; 120(7):2537‑48. < PMID: 20516639 >