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CyFlow™ NTAL (LAB) PE

CyFlow™ NTAL (LAB) PE
Antibody: Yes
Antigen: NTAL (LAB)
Application: Flow cytometry
Clonality: monoclonal
Clone: NAP-07
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Adaptor proteins
Format/Fluorochrome: PE
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Mouse
Product number: AC648454

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ NTAL (LAB) PE
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenRecombinant cytoplasmic domain (aa 91-243) of human NTAL
Background InformationNTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 3.3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsi V: Non‑T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med. 2002 Dec 16; 196(12):1617‑26. < PMID: 12486104 > | Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM: NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood. 2004 Jul 1; 104(1):207‑14. < PMID: 15010370 > | Volna P, Lebduska P, Draberova L, Simova S, Heneberg P, Boubelik M, Bugajev V, Malissen B, Wilson BS, Horejsi V, Malissen M, Draber P: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18; 200(8):1001‑13. < PMID: 15477348 > | Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T: LAT and NTAL mediate immunoglobulin E‑induced sustained extracellularsignal‑regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun; 27(12):4406‑15. < PMID: 17420272 > | Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non‑adherent mast cells. J Immunol Methods. 2007 Dec 1; 328(1‑2):139‑51. < PMID: 17900607 >

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