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CyFlow™ Porcine acrosin Purified

CyFlow™ Porcine acrosin Purified
Antibody: Yes
Antigen: Porcine acrosin
Application: Flow cytometry, Immunocytochemistry, Western blot
Clonality: monoclonal
Clone: ACR-2
Field of Interest: Fertility & Reproduction
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Pig
Product number: CP171824

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ Porcine acrosin Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenAcid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-α benzoylarginine-4-nitroanilide was used for immunization
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Peknicova J, Moos J: Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct; 22(5):427‑35. < PMID: 2073053 > | Moos J, Tesarik J, Leca G, Peknicova J: Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2; 294(1‑2):27‑30. < PMID: 1743288 > | Moos J, Peknicova J, Tesarik J: Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore‑induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16; 1176(3):199‑207. < PMID: 8471622 > | Moos J, Peknicova J, Tesarik J: Protein‑protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug; 49(2):408‑15. < PMID: 8373968 > | Peknicová J, et al.: Anim Reprod Sci. 1994; 35:255. < No PMID > | Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M: Monoclonal antibodies to intra‑acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15; 56(2):211‑23. < PMID: 11480614 > | Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J: Effect of estrogens on boar sperm capacitation in vitro. Reprod Biol Endocrinol. 2010 Jul 13; 8:87. < PMID: 20626847 >

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