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CyFlow™ Perforin PE

CyFlow™ Perforin PE
Antibody: Yes
Antigen: Perforin
Application: Flow cytometry
Clonality: monoclonal
Clone: dG9
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Apoptosis
Format/Fluorochrome: PE
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Cow, Human
Product number: AC676621

For Research Use Only

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Description
Quantity 100 tests Volume 1.0 mL Immunogen Purified granules from human YT lymphoma... more
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CyFlow™ Perforin PE

Quantity100 tests
Volume1.0 mL
ImmunogenPurified granules from human YT lymphoma cell line
Background InformationPerforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Hersperger AR1, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov; 73(11):1050‑7. < PMID: 18615597 > | Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell‑in‑cell structures formed between human cancer cell lines and the cytotoxic regulatory T‑cell line HOZOT. J Mol Cell Biol. 2010 Jun; 2(3):139‑51. < PMID: 20348186 > | Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(‑) subpopulation of macaque circulatory natural killer cells can mediate both antibody‑dependent and antibody‑independent cytotoxic activities. Immunology. 2011 Nov; 134(3):326‑40. < PMID: 21978002 > | Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim‑3‑expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti‑TB effector functions. PLoS Pathog. 2012; 8(11):e1002984. < PMID: 23144609 >

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