CyFlow™ TCR alpha/beta PE
| Antibody: | Yes |
| Antigen: | TCR α/β |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | IP26 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | PE |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BF526310 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Not available |
| Background Information | The antigen-specific T cell receptor (TCR) is composed of either α and β subunit, or γ and δ subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR α/β heterodimers, whereas the T cells expressing TCR γ/δ heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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