CyFlow™ TCR gamma/delta PE
| Antibody: | Yes |
| Antigen: | TCR γ/δ |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | V65 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Immunophenotyping, MHC |
| Format/Fluorochrome: | PE |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Rat |
| Product number: | AC827019 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 0,5 |
| Quantity | 0.1 mg |
| Volume | 0.2 mL |
| Immunogen | TCR α/β-negative CD3-positive rat T cell hybridoma III.89.1.4 line |
| Background Information | The antigen-specific T cell receptor (TCR) is composed of either α and β subunit, or γ and δ subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR α/β heterodimers, whereas the T cells expressing TCR γ/δ heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression. |
| Usage | The reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Giezeman-Smits KM, Jonges LE, Chambers WH, Brisette-Storkus CS, Van Vlierberghe RL, Van Eendenburg JD, Eggermont AM, Fleuren GJ, Kuppen PJ: Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL‑2‑activated rat NK cells. J Leukoc Biol. 1998 Feb; 63(2):209‑15. < PMID: 9468279 > | Tam FW, Smith J, Morel D, Karkar AM, Thompson EM, Cook HT, Pusey CD: Development of scarring and renal failure in a rat model of crescentic glomerulonephritis. Nephrol Dial Transplant. 1999 Jul; 14(7):1658‑66. < PMID: 10435873 > | Hosea HJ, Rector ES, Taylor CG: Zinc‑deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood. J Nutr. 2003 Dec; 133(12):4239‑42. < PMID: 14652378 > | Xystrakis E, Dejean AS, Bernard I, Druet P, Liblau R, Gonzalez-Dunia D, Saoudi A: Identification of a novel natural regulatory CD8 T‑cell subset and analysis of its mechanism of regulation. Blood. 2004 Nov 15; 104(10):3294‑301. < PMID: 15271801 > | Henriksson G, Helgeland L, Midtvedt T, Stierna P, Brandtzaeg P: Immune response to Mycoplasma pulmonis in nasal mucosa is modulated by the normal microbiota. Am J Respir Cell Mol Biol. 2004 Dec; 31(6):657‑62. < PMID: 15347559 > | van den Brandt J, Kwon SH, Hünig T, McPherson KG, Reichardt HM: Sustained pre‑TCR expression in Notch1IC‑transgenic rats impairs T cell maturation and selection. J Immunol. 2005 Jun 15; 174(12):7845‑52. < PMID: 15944289 > | Moghaddami M, Cleland LG, Mayrhofer G: MHC II+ CD45+ cells from synovium‑rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro. Int Immunol. 2005 Aug; 17(8):1103‑15. < PMID: 16030130 > | De Bernardis F, Lucciarini R, Boccanera M, Amantini C, Arancia S, Morrone S, Mosca M, Cassone A, Santoni G: Phenotypic and functional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis. Infect Immun. 2006 Jul; 74(7):4282‑94. < PMID: 16790803 >