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CyFlow™ TNF-alpha APC

CyFlow™ TNF-alpha APC
Antibody: Yes
Antigen: TNF-α
Application: Flow cytometry
Clonality: monoclonal
Clone: MAb11
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Signaling
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates, Pig
Product number: AF800796

For Research Use Only

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Description
Quantity 100 tests Volume 1.0 mL Immunogen Recombinant human TNF α Background... more
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CyFlow™ TNF-alpha APC
Quantity100 tests
Volume1.0 mL
ImmunogenRecombinant human TNF α
Background InformationTNF α is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF α easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Wahlström J, Katchar K, Wigzell H, Olerup O, Eklund A, Grunewald J: Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001 Jan; 163(1):115‑21. < PMID: 11208635 > | Visser J, Graffelman W, Blauw B, Haspels I, Lentjes E, de Kloet ER, Nagelkerken L: LPS‑induced IL‑10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone. J Neuroimmunol. 2001 Oct 1; 119(2):343‑9. < PMID: 11585638 > | Attarbaschi T, Willheim M, Ramharter M, Hofmann A, Wahl K, Winkler H, Graninger W, Winkler S: T cell cytokine profile during primary Epstein‑Barr virus infection (infectious mononucleosis). Eur Cytokine Netw. 2003 Jan-Mar; 14(1):34‑9. < PMID: 12799212 > | Yan SR, Qing G, Byers DM, Stadnyk AW, Al-Hertani W, Bortolussi R: Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide. Infect Immun. 2004 Mar; 72(3):1223‑9. < PMID: 14977922 > | Cesaro-Tadic S, Dernick G, Juncker D, Buurman G, Kropshofer H, Michel B, Fattinger C, Delamarche E: High‑sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems. Lab Chip. 2004 Dec; 4(6):563‑9. < PMID: 15570366 >

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