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CyFlow™ Kappa-LC Pacific Blue™

CyFlow™ Kappa-LC Pacific Blue™
Antibody: Yes
Antigen: κ light chains
Application: Flow cytometry
Clonality: monoclonal
Clone: A8B5
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Field of Interest: Immunoglobulins
Format/Fluorochrome: Pacific Blue™
Isotype: IgG1
Laser: Violet
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BF605953

For Research Use Only

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Not available Background Information... more
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CyFlow™ Kappa-LC Pacific Blue™

Quantity100 tests
Volume0.4 mL
ImmunogenNot available
Background InformationImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (κ and λ; both having a molecular weight of 22.5 kDa). κ and λ consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of κ to λ is 70:30.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975; 194:77‑95. < PMID: 47690 > | Diaz de Domingo NB, Lardo MM, Romero Artaza J, Merelli A, Carbia CD, Sánchez Avalos JC: Aplicacion de tecnicas inmunocitoquimicas para el diagnostico de neoplasias hematologicas. Medicina (B Aires). 1999; 59(1):11‑6. < PMID: 10349112 > | Konig A, Wennemuth G, Soyer HP, Hoffmann R, Happle R, Krause W: Vulvar amyloidosis mimicking giant condylomata acuminata in a patient with multiple myeloma. Eur J Dermatol. 1999 Jan-Feb; 9(1):29‑31. < PMID: 9920983 > | Scheeren FA, Diehl SA, Smit LA, Beaumont T, Naspetti M, Bende RJ, Blom B, Karube K, Ohshima K, van Noesel CJ, Spits H: IL‑21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis. Blood. 2008 May 1; 111(9):4706‑15. < PMID: 18296629 > | Di Bartolo DL, Hyjek E, Keller S, Guasparri I, Deng H, Sun R, Chadburn A, Knowles DM, Cesarman E: Role of defective Oct‑2 and OCA‑B expression in immunoglobulin production and Kaposi's sarcoma‑associated herpesvirus lytic reactivation in primary effusion lymphoma. J Virol. 2009 May; 83(9):4308‑15. < PMID: 19224997 > | Avilés-Salas A, Chanona-Vilchis JG, Candelaria-Hernández MG, Peña-Torres Mde L, Cuéllar-Mendoza ME: Presentación inicial de un linfoma plasmablástico como tumor ovárico. Ginecol Obstet Mex. 2012 Jun; 80(6):430‑4. < PMID: 22826973 >

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