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CyFlow™ Kappa-LC Pacific Blue™

CyFlow™ Kappa-LC Pacific Blue™
Antibody: Yes
Antigen: κ light chains
Application: Flow cytometry
Clonality: monoclonal
Clone: TB28-2
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Field of Interest: Immunoglobulins
Format/Fluorochrome: Pacific Blue™
Isotype: IgG1
Laser: Violet
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BW314376

For Research Use Only

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Human IgG-κ myeloma protein Background... more
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CyFlow™ Kappa-LC Pacific Blue™

Quantity100 tests
Volume0.4 mL
ImmunogenHuman IgG-κ myeloma protein
Background InformationImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (κ and λ; both having a molecular weight of 22.5 kDa). κ and λ consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of κ to λ is 60:40.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti‑Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987 Jun 15; 138(12):4150‑8. < PMID: 3495581 > | Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin‑associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci USA. 1992 Sep 15; 89(18):8522‑6. < PMID: 1382292 > | Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome: An immunophenotypic analysis. Am J Clin Pathol. 2001 Aug; 116(2):204‑10. < PMID: 11488066 > | Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C: EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and significance of four‑color flow cytometry compared to consensus IGH‑polymerase chain reaction at initial staging and for follow‑up examinations. Haematologica. 2008 Apr; 93(4):551‑9. < PMID: 18379010 > | Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts andplasma cells including detailed phenotypic and molecular characterization. Blood. 2009 Dec 10; 114(25):5173‑81. < PMID: 19846886 >

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