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CyFlow™ Lambda-LC FITC

CyFlow™ Lambda-LC FITC
Antibody: Yes
Antigen: λ light chains
Application: Flow cytometry
Clonality: monoclonal
Clone: 1-155-2
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunoglobulins
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AR685249

For Research Use Only

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Human IgA1 λ myeloma protein... more
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CyFlow™ Lambda-LC FITC

Quantity100 tests
Volume0.4 mL
ImmunogenHuman IgA1 λ myeloma protein
Background InformationImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (κ and λ; both having a molecular weight of 22.5 kDa). κ and λ consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of κ to λ is 60:40.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kubagawa H, Gathings WE, Levitt D, Kearney JF, Cooper MD: Immunoglobulin isotype expression of normal pre‑B cells as determined by immunofluorescence. J Clin Immunol. 1982 Oct; 2(4):264‑9. < PMID: 6815220 > | Bahler DW, Pindzola JA, Swerdlow SH: Splenic marginal zone lymphomas appear to originate from different B cell types. Am J Pathol. 2002 Jul; 161(1):81‑8. < PMID: 12107092 > | Tierens AM, Holte H, Warsame A, Ikonomou IM, Wang J, Chan WC, Delabie J: Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B‑cell lymphoma of activated B‑cell type but not of germinal center B‑cell type. Haematologica. 2010 Aug; 95(8):1334‑41. < PMID: 20145271 >

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